In order to monitor membrane wetting, break through or the quality of permeate, permeate conductivity was monitored continuously using a conductivity-meter, VWR, Radnor, PA, USA. A polycarbonate membrane module with 33.75 cm 2 effective surface area was employed in present investigation.
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Proteins were then transferred to a nitrocellulose membrane (Hybond ECL, GE Healthcare Life Sciences, Freiburg, Germany) using wet blotting (0.7 mA/cm 2, 90 min). Membrane was blocked with 5% low fat milk in TBS/Tween 0.1% and incubated with primary antibody diluted with 5% low fat milk in TBS/Tween 0.1% or 5% BSA in TBS/Tween 0.1% for 1 h at RT or overnight at 4°C.
2017/1/25 · The sample was drawn into a syringe and filtered with a 0.45-μm syringe filter (VWR International 13 mm with PTFE membrane) in order to avoid dust particles. The exception to filtering step was made with the bigger hollow MSNs in which case the diluted suspension was used as prepared.
a) Cross‐section of the multilayers (double arrow) lying on PTFE membrane as visualized by light microscopy; HES stain; bar = 20 µm. b) Cross‐section of the upper layer as visualized by transmission electron microscopy: tight junction (arrow) between two cells; (N: nucleus) at the upper layer; one of the cells displays apical microvilli (*); bar = 500 nm.
Prepare transfer membrane (PVDF). Immerse in 100% methanol for 1–2 sec to hydrate it. 2. Equilibrate the membrane in transfer buffer for 10 min. 3. Equilibrate the separating gel with transfer buffer for 10 min. 4. Place 3 sheets of filter paper saturated with 5. 6.
Polyvinylidene difluoride (PVDF) membrane is ideal for western blotting applications as well as for amino acid analysis and protein sequencing of small amounts of proteins (as little as 10 pmoles). In addition, PVDF membranes can be used, stripped and reprobed without a
Hyper-cross-linked thin-film composite membranes were fabricated on the PAN ultrafiltration membrane (GMT GmbH, Rheinfelden, Germany) as supports via interfacial polymerization. First, the supports with the proper area were fixed with PTFE frames. 12 ml of
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If you’re creating new molecules in your pharmaceutical or chemical research or university laboratory, VWR offers an extensive range of products to help you with your discoveries. Production Spoločnosť VWR vám dokáže poskytnúť podporu vo vašom prostredí počnúc surovinami, až po kontrolu hygieny
5. Shake well, and filter the extract through a 0.2 μm PTFE membrane syringe filter (p/n ). 6. If necessary, dilute to the desired concentration with acetonitrile. Mobile Phase Preparation Ammonium formate was weighed and diluted to 10 mM
