1/8/2019 · Each sample was centrifuged at 3000 rpm for 5 min and filtered with a 0.22 μm polytetrafluoroethylene (PTFE) membrane filter. The resulting solution was used for the microextraction procedures. For juice samples, a 3 mL aliquot was added to a 15 mL centrifuge tube and centrifuged at 5000 rpm for 15 min.