Hydrophilic PVDF Membrane Exhibits Extremely Low Protein Binding 2.5 L of PBS containing 100 µg/mL BSA and approx 100k cpm/mL 125 I-labeled BSA was perfused through 13 mm discs. After perfusion, discs are blotted on paper towels to remove excess liquid and are then placed in a gamma counter. µg/cm 2 was calculated from cpm/discs.