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Then, 110 μl of the IMS-purified sample was passed through a hydrophilic polytetrafluoroethylene (PTFE) membrane (pore size 1.0 μm, diameter 25 mm; Advantec), followed by fluorescent staining of protozoa on a membrane using EasyStain (BTF, North Ryde
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The formed precipitates were collected by filtration using a 1.0 μm pore-sized PTFE membrane filter and washed with a mixture of 1:3 dry MeOH/dry CH 2 Cl 2 (8.00 mL). A solution of Ac 2 O (3.56 g, 34.9 mmol) in dry MeOH (66.0 mL) was added to the obtained solid, and the resulting mixture was stirred overnight at room temperature and then evaporated.
The purified oocysts were stained on hydrophilic PTFE membrane filters (diameter, 25 mm; pore size, 0.2 μm; Advantec, Inc.) with a vacuum filtration unit (Advantec, Inc.). Staining of the oocysts was performed with a commercial kit according to the instructions (Hydrofluoro Combo
The PTFE-based PFAEMs also showed excellent oxidative stabilities in a highly acidic vanadium solution, which were superior to that of the commercial AMX membrane. Consequently, through this study, high-performance AEMs capable of long-term use under harsh alkaline and acidic conditions have been developed through the combination of porous PTFE substrates and an ionomer having both a high
2011/1/15 · At t = 0, the gas beneath the PTFE membrane was switched to 10 or 1% NO (350 sccm) and the O 2 and NO concentrations were monitored until steady values were obtained. For experiments at subambient O 2 levels, pure N 2 was flowed through both the PDMS tubing and the reactor base until the desired P ¯ O 2 was reached, at which time the gas streams were switched to the appropriate O 2 and
Following 10 min of ultrasound irradiation, 10 min of shaking, and another 10 min of ultrasound irradiation, the solution was filtered through 0.45 μm membrane filter (PTFE, Advantec, Tokyo, Japan). The amounts of each compound in the extract were measured using HPLC according to
There is provided a membrane electrophoresis which renders possible glycan analysis of polysaccharide and glycoprotein by separating protein, glycoprotein or polysaccharide by electrophoresis, followed by carrying out a glycan-releasing treatment of the
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2018/5/17 · Adherent, proliferating cells were then treated with 1 ml of sterile filtered (Advantec PTFE membrane filter; pore size 0.2 μM) auric chloride (HAuCl 4) (10 −3 M) solution prepared in PBS and incubated at 37 C in a humidified 5% CO 2 atmosphere.
0.22 µm PTFE membrane filter (Advantec, Tokyo, Japan). The 20 µl MeOH extracts of BS-99, BS-23857 and BS-33608 were injected into a reverse phase HPLC column (RP-18 column, 5 µm, 4 × 250 mm; Merck) for detecting Iturin A and surfactin. A
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