diode detector, C-18 detector, Vortex mixing instrument, High speed centrifuge, Ultrasonicator, Nylon syringe filter (0.22 um). High performance liquid chromatography conditions were Mobile phase- Acetonitrile: Aq. 0.1% acetic acid, 50:50 (v/v), Flow rate 1.0 ml/min, column temperature 25 °C and Photo diode detector.