0.22um Filter at Thomas Scientific. Foxx Life Sciences. and validate. All EZBio® “MTO” Single Use Media Bottle Assembly, include, Bottle, Cap, Cap Insert, Tubing, HB Fittings, a Diptube, for accurate draw, a 0.22um Vent filter and a Ratchet Style Pinch Clamp. Each finished is double bagged and Gamma Sterilized in the US.